Synthesis and Antimicrobial Studies of Some Novel Mannich Bases Derived from Secondary Amines

The amino alkylation of aromatic substrates by the Mannich reaction is of considerable importance for the synthesis and modification of biologically active compounds. The Mannich derivatives of 2-Chloro 4nitrobenzamide with formaldehyde and various secondary amines were synthesized for study of their biological effects. The structures of the synthesized compounds were assigned on the basis of elemental analyses, UV, IR and HNMR spectral studies. The biological screenings of these synthesized compounds were done against some Gram-positive and Gram-negative bacteria with a view to explore their antimicrobial action by disc diffusion method at 40, 80 and 160 mg/ml respectively. The results reveal the potential and importance of mounting new Mannich bases against pathogens under investigation and found to be low toxic as ascertained by LD50 test.